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. 2021 Sep 3;10(18):e020521. doi: 10.1161/JAHA.120.020521

Figure 2. Induction by serum of Egr‐1 in CRISPR/Cas9 clones.

Figure 2

Growth‐quiescent wild‐type (WT) (WT1, WT2, WT3), mutant (M) (M26A, M26B, M26C), and deletion (DEL) (DEL1, DEL2, DEL3) cells were treated with (+) or without (−) 5% FBS for 1 hour. Upper panels show total Egr‐1 protein and lower panels correspond to α‐tubulin. Approximate molecular weight markers are shown. Immunoblots are representative of 2 biologically independent experiments. Egr‐1 indicates early growth response‐1; and IB, immunoblotting.