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. 2021 Sep 3;10(18):e020521. doi: 10.1161/JAHA.120.020521

Figure 4. Effect of Ser26 mutation and Egr‐1 deletion on real‐time endothelial cell growth and metabolic activity.

Figure 4

A, Cell growth assays were performed using the xCELLigence system and WT1, M26A, and DEL1 cells. Data represent the means of the means of 3 biologically independent experiments±SEM. Left, representative xCELLigence real‐time profile. Right, combined means 96 hours after seeding. Significance was assessed by 1‐way ANOVA. ***P<0.001. B, MTT assays were performed with CRISPR/Cas9 clones WT1, WT2, WT3, M26A, M26B, M26C, DEL1, DEL2, and DEL3. Data represent the means of the means of 3 biologically independent experiments±SEM. Left, means of individual experiments. Right, combined means. Significance was assessed by Mann‐Whitney or t test. *P<0.05. ****P<0.0001. SFM denotes serum free medium; FBS denotes medium containing fetal bovine serum. DEL indicates deletion; Egr‐1, early growth response‐1; M, mutant; ns, not significant; WT, wild‐type; and OD, optical density.