Figure 6. Effect of Ser26 mutation and Egr‐1 deletion on endothelial network formation on Matrigel.

A, Network formation assays were performed with CRISPR/Cas9 clones WT1, M26A, and DEL1 in MCDB131 containing 1% FBS and 50 ng/mL FGF‐2 (fibroblast growth factor‐2) on a bed of Matrigel. Data represent the means of the means of 3 biologically independent experiments±SEM. Significance was assessed by 1‐way ANOVA. **P<0.01. B, Representative network formation. The cells were photographed under 40× magnification. Three separate fields are shown for each cell type. DEL indicates deletion; Egr‐1; early growth response‐1; M, mutant; ns, not significant; and WT, wild‐type.