Figure 7. Effect of Ser26 mutation and Egr‐1 deletion on endothelial early and late apoptosis.

A, Annexin V‐FITC⁺/PI^‐ (left) or annexin V‐FITC⁺/PI⁺ (right) staining comparing CRISPR/Cas9 clones WT1, M26A, and DEL1 in MCDB131 containing 10% FBS. Data represent the means of the means of 3 biologically independent experiments±SEM. Significance was assessed by 1‐way ANOVA. ***P<0.001; ****P<0.0001. B, Representative annexin V‐FITC⁺/PI^‐ and annexin V‐FITC⁺/PI⁺ staining with WT1, M26A, and DEL1 cells by flow cytometry. Annexin V‐FITC⁺/PI^‐ and annexin V‐FITC⁺/PI⁺ reflect early and late apoptosis, respectively. DEL indicates deletion; Egr‐1, early growth response‐1; M, mutant; ns, not significant; and WT, wild‐type.