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. 2021 Sep 6;10(18):e021140. doi: 10.1161/JAHA.121.021140

Figure 2. Decrease in coronary endothelial cell (CEC)s, endothelial nitric oxide (eNOS) and eNOS phosphorylation in type‐1 diabetic hearts when compared between ALDH2*2 mutant and WT control mice.

Figure 2

Representative micrographs of CD31+ immunostaining of CECs of cardiac sections (A through D) and the quantification of the number of CECs in cardiac sections (E) were shown from WT and ALDH2*2 control (Ctrl.) and diabetic mellitus (DM) mice. Quantification data of myocardial coronary perfusion pressure (F) were shown from WT and ALDH2*2 Ctrl. and DM mice. Data are presented as mean±SEM. Representative micrographs of double staining of CD31+ and endothelial nitric‐oxide synthase (eNOS) immunofluorescence staining in CECs of cardiac sections were shown from WT and ALDH2*2 Ctrl. and DM (G through R). The quantification data of CD31+ cells (S) and eNOS + cells (T) were shown. Representative Western blot images of eNOS, phospho eNOS (p‐eNOS), and porin levels (U) and eNOS quantification data (V), phospho eNOS S1177 (W), and the ratio of phospho eNOS S1177/eNOS (X) were shown. Data are presented as mean±SEM. *P<0.05, **P<0.01, and ***P<0.001. N=6. ALDH2*2 indicates aldehyde dehydrogenase 2 allele; and WT, wild type.