TABLE 4.
Technical aspects and costs per slide for each fixation and staining procedure
| Procedure | Fixative(s) used | No. of steps | Centrifugation time (min) | Slide preparation time (min)a | Staining method | Staining time (min) | Fixation and staining costs ($) (reagents only) |
|---|---|---|---|---|---|---|---|
| PVA | Mercuric chloride | 16 | 10 | 15 | Wheatley's trichrome | 40 | 3.36 |
| EC | Zinc sulfate | 15 | 10 | 14 | EcoStain | 15 | 3.92 |
| PS | Ethanol bis-carbonyl compounds | 22 | 3 | 8 | Modified Wheatley's trichrome | 23 | 2.76 |
| PF | Ethanol, methanol, isopropanol, CH2O | 21 | 2 | 11 | Trichrome plus | 13 | 3.28 |
a
Including cenrifugation time.