Figure 5.

HOTAIR inhibited the expression of UBXN1 by epigenetic modification. (A) ChIP assay detected the H3K27me3 modification on the promotor region of UBXN1. (B) Upper: ChIRP assay enriches HOTAIR RNA with high yield and specificity. Lower: ChIRP assay detect the HOTAIR binding region on the promotor of UBXN1. (C) ChIP assay detected NF-κB binding on the promotor of CD274 in U87 treated with AQB or vehicle as control. (D) ChIP assay detected NF-κB binding on the promotor of HOTAIR in U87 cells treated with AQB or vehicle as control. (E) T cell killing assay of TBD cells treated with siRNAs for EZH2 and HOTAIR. Cell nuclei were stained by DAPI in blue, and apoptotic cells were displayed by fluorescent products of caspase 3/7 cleavage (green). For all the statistical histograms above, values are means ± s.d. from n = 3 independent experiments. The P value was determined by two-sided Student’s t-test.