FIG 5.

Antibody responses during long-term heterologous prime-boost vaccination study. Mice were immunized with Ad5-YFV and LMA vaccines in 2-dose (prime-boost) regimens in which Ad5-YFV and LMA were administered 21 days apart in various combinations. The composition of the groups and the experiment time course are depicted in panels A and B, respectively. Sera were collected on day 63 of the study, which was 42 days after the 2nd vaccination dose. The total IgG and its isotype IgG1/IgG2a titers specific to F1-V were determined by ELISA and are displayed in panels C and D, respectively, while the total IgG titers specific to individual antigens F1, LcrV, and YscF are shown in panel E. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test (A, C, and D) and by two-way ANOVA with Tukey’s post hoc test (B). Asterisks directly above bars indicated significance compared to the control group, while asterisks with comparison bars denoted significance between the indicated groups. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Two biological replicates were performed and data plotted. In vitro studies had 3 replicates.