FIG 9.

Splenocyte proinflammatory and anti-proinflammatory responses during long-term heterologous prime-boost vaccination study. The splenocytes isolated from mice described for Fig. 6 (postimmunization) and Fig. 8 (post-CO92 challenge) were further stimulated with rF1-V (100 μg/ml) for 3 days. The cytokines in the culture supernatants were analyzed by using Bioplex-23 assay and expressed as the arithmetic means ± standard deviations. The proinflammatory and anti-proinflammatory cytokines are shown. Statistical significance was determined using two-way ANOVA with Tukey’s post hoc test to compare multiple time points or Student's t test to compare 2 groups within the same time point. Asterisks above columns represented comparison to the control group, while horizontal bars represented differences between test groups. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Two biological replicates were performed and data plotted. In vitro studies had 3 replicates.