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. 2021 Dec 7;12(6):e03075-21. doi: 10.1128/mBio.03075-21

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Copyright © 2021 Yao et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — Analysis of the effects of the top 10 RSCs on RSV RNA levels in infected A549 cells and the subcellular localization of the 10 RSCs. (A) RSV RNA levels in infected A549 cells (MOI = 1) transfected with three pooled siRNAs for each circRNA (siCircRNA), with the exception of RSC2, assessed by qRT-PCR. (B) qRT-PCR analysis of the respective circRNAs in A549 cells treated with three pooled siRNAs as described above for panel A. In panels A and B, the RSV RNA levels or circRNA levels were normalized to the negative-control siRNA (siNC) group. (C) qRT-PCR data indicating the abundances of 10 RSCs in the subcellular localization (cytoplasm or nucleus) of A549 cells. The amounts of circRNAs were normalized to the value measured in the nucleus. C/N ratio, ratio of cytoplasm to nucleus.