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. 2021 Nov 8;13(12):e14397. doi: 10.15252/emmm.202114397

Figure EV3. BN‐PAGE of multiple mouse tissues show increased staining for large supercomplex (SXL) in Uqcrh −/− mice.

Figure EV3

  1. Mitochondrial membranes from kidney samples of wild‐type (WT) and Uqcrh −/− mice were solubilised with digitonin and separated on native gradient gels. Protein complexes were stained with Coomassie (left panel) with NADH:NTB reductase activity stain (centre panel) and with an antibody against Core II subunit of complex III (Uqcrc2, right panel).
  2. Mitochondrial membranes from brain samples of wild‐type (WT) and Uqcrh −/− mice were solubilised with digitonin and separated on native gradient gels. Protein complexes were stained with Coomassie (left panel) with NADH:NTB reductase activity stain (right panel).
  3. Mitochondrial membranes from skeletal muscle samples of wild‐type (WT) and Uqcrh −/− mice were solubilised with digitonin and separated on native gradient gels. Protein complexes were stained with Coomassie (left panel) with NADH:NTB reductase activity stain (right panel).
  4. Mitochondrial membranes from liver samples of wild‐type (WT) and Uqcrh −/− mice were solubilised with digitonin and separated on native gradient gels. Protein complexes were stained with Coomassie (left panel) with NADH:NTB reductase activity stain (right panel).

Data information: Assignment of complexes: I, complex I; III2, complex III dimer; IV, complex IV; S0, supercomplex containing complex I and a dimer of complex III; S1, supercomplex containing complex I, a dimer of complex III and 1 copy of complex IV, SXL, Supercomplex or Megacomplex containing I, III and higher signals also additional copies of IV.