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. 2021 Nov 9;13(12):e14072. doi: 10.15252/emmm.202114072

Figure 4. EBV‐LMP1 fixes ANT1 at the m‐state to inhibit ADP/ATP exchange.

Figure 4

  • A, B
    The ADP/ATP exchange rate in EBV‐LMP1‐positive nasopharyngeal carcinoma cells is decreased. The fluorescence value of Magnesium Green™ (1 μM) was measured at 505 nm (Ex)/535 nm (Em) by enzymography (A), and the ADP/ATP exchange rate was calculated after conversion to ATP (B). Data are presented as means ± SEM (paired t‐test, n = 5, biological replicates per group, **P < 0.01).
  • C
    LMP1‐negative or LMP1‐positive NPC cells were treated with BKA (1 μM) or CATR (5 μM) for 24 h, mitochondrial OCR assessed with the XF24 extracellular flux analyzer (Seahorse) under sequential treatment with oligomycin, FCCP, and antimycin A. Data are presented as means ± SEM (n = 6, biological replicates per group).
  • D
    Oxidative phosphorylation‐related metrics based on OCR data. Data are presented as means ± SEM (paired t‐test, n = 6, biological replicates per group, *P < 0.05,**P < 0.01).