Fig. 3.

Cells that bypassed-OIS show increased readthrough. (A) RT-qPCR for H-RAS and senescent marker mRNAs were performed in IMR-90 cells at day 12 and 35 post infection with an empty vector (Vector) or H-RasV12 oncogene (H-RasV12). Data are normalized over TBP and HMBS, and presented as means relative to vector infected cells, n=3. B. Luciferase activities in non-senescent and senescent cells measured at day 12 and 35 post-infection in cells having the indicated reporters. Normalizations are presented as means relative to vector-infected cells from three independent experiments with technical triplicates for each experiment, except for UGA reporter, which are representative of two independent experiments with similar results. Error bars indicate SD of biological triplicates (AQP4, HPN, VASP) or technical triplicates (UGA). *=P<0.05, **=P<0.01 are significantly different, using two-tailed Student's t-test.