REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
LentiBrite Histone-H2B-GFP Lentiviral Biosensor	Merck Millipore	Cat#17-10229
Chemicals, peptides, and recombinant proteins
DMEM	Gibco	Cat#10569-010
McCoy’s 5A (Modified) Media	Gibco	Cat#16600-082
0.40% Trypan Blue	Bio-Rad Laboratories	Cat#1450013
Trypsin:EDTA Solution - 0.05%	Gemini Bio Products	Cat#400-150
DPBS (1×)	Gibco	Cat#14190-144
Penicillin-Streptomycin	Gemini Bio Products	Cat#400109
Fetal Bovine Serum	Gemini Bio Products	Cat#100-500
Rat Tail Collagen Type I	Corning	Cat#354249
Matrigel	Corning	Cat#356231
Critical commercial assays
Organ on Chip	Emulate, Inc	Basic Research Kit
Experimental models: Cell lines
Human: RFP expressing Human umbilical vein endothelial cells	ANGIO-PROTEOMIE	Cat# cAP-0001RFP
Human: Caco2 C2BBe1	ATCC	Cat# CRL-2102
Human: HCT116	ATCC	Cat# CCL-247
Software and algorithms
Harmony High-Content Imaging and Analysis Software	PerkinElmer	N/A
Imaris image analysis	Imaris	N/A
GraphPad Prism 8	GraphPad Software, Inc.	https://www.graphpad.com/
Biorender	BioRender	https://biorender.com/
Other
Zoë Culture Module	Emulate, Inc.	N/A
Orb Hub Module	Emulate, Inc.	N/A
Perkin Elmer Operetta High Content Screening (HCS) platform	PerkinElmer	N/A
Olympus FV3000	OLYMPUS	N/A
TC20 Automated Cell Counter	Bio-Rad Laboratories	N/A