Figure 2.

The figure represents the different ex vivo models. The Using chamber or the inTESTine™ system works by removing a segment of intestinal mucosa from an animal, which is then mounted between two halves of a chamber filled with physiological buffer to simulate the mucosal and basolateral passage flow. In everted intestinal rings, the animal's intestine is cut into ring slices and transferred into oxygenated culture media. This model is a useful tool for investigating uptake into intestinal cells and metabolism in different regions of the intestine—Created with BioRender.com.