FIGURE 1.

Oligodendroglial cell death rates upon L48H37, compound 20, and folimycin treatment. Immunocytochemical staining by means of cleaved caspase 3 (CC-3) within OPCs after 1 day revealed high doses of L48H37, folimycin, and compound 20 led to an increase in the percentage of apoptotic OPC as judged by the expression of activated CC-3 (A,C,E). DMSO was used as a control and was applied at equal dilutions. Representative pictures show CC3 positive cells marked via immunocytochemical staining and DAPI-stained nuclei (B,D,F). Arrows represent CC3 positive cells. Scale bar: 20 μm. Data are shown as mean values (± SEM) and derived from n = 3 experiments. Statistical significance was calculated using one-way ANOVA with Tukey post-test (ns, not significant, *p < 0.05, **p < 0.01, and ***p < 0.001). DMSO, dimethylsulfoxide; DAPI, 4′,6-diamidin-2-phenylindol.