FIGURE 2.

Gene expression alterations due to proinflammatory effects of the recombinant envelope protein (ENV) on cultured rat oligodendroglial precursor cells. (A,B) Quantitative RT-PCR after 1d revealed that upon ENV protein stimulation a significant upregulation of iNOS and IL-6 transcript levels occurred, which was compensated to control transcript levels upon L48H37 or compound 20 treatment. Data are shown as mean, and error bars represent SEM. A number of experiments: n = 4. Statistical significance was calculated using one-way ANOVA with Tukey post-test (ns, not significant, *p < 0.05, **p < 0.01, and ***p < 0.001). iNOS, inducible nitric oxide synthase; IL-6, interleukin-6.