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. 2021 Nov 23;15:777542. doi: 10.3389/fncel.2021.777542

FIGURE 4.

FIGURE 4

L48H37 and compound 20-mediated rescue of ENV-dependent oligodendroglial myelinating deficits. Quantification of MBP-positive oligodendrocytes (at DIV26) upon ENV protein or ENV protein plus L48H37 (A), folimycin (B) or compound 20 (C) exposure, starting at DIV17. Representative in vitro myelinating co-cultures displaying the rescue of myelinating oligodendrocytes (asterisks; MBP immunostaining signals in green) by means of substance application in presence of ENV protein (axons are visualized by means of NF staining in blue, cell nuclei are marked by Olig2 in red). Scale bars, 50 μM. Inserts in the top right corners correspond to enlarged fields marked by the white squares. Data are shown as mean as values, error bars represent SEM. Number of experiments: n = 3. Statistical significance was calculated using one-way ANOVA with Tukey post-test (ns, not significant, **p < 0.01, ***p < 0.001). DAPI, 4′,6-diamidin-2-phenylindol; MBP, myelin basic protein; OPCs, oligodendroglial precursor cells; DMSO, dimethylsulfoxide; ENV, envelope protein.