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. 2021 Nov 16;25(24):11310–11321. doi: 10.1111/jcmm.17062

FIGURE 5.

FIGURE 5

NMP attenuates ischaemia‐reperfusion injury in DCD livers. The competitive CIRP inhibitor C23 (300 ng/ml) was mixed into the perfusate of the HOPE group. The livers of the four groups were re‐perfused using the NMP system for 2 h (n = 5). (A) HE staining, CD68, and MPO immunohistochemistry of representative liver sections (magnification 200×). (B) Liver injury in the CS I/R and HOPE I/R groups was more severe than that in the NMP I/R group. (C) The number of CD68‐positive cells and (D) MPO‐positive cells in each group. (E) TUNEL staining. (F) The number of TUNEL‐positive cells in each group. (G) ALT levels in the perfusate of the four groups. (H) AST levels in the perfusate of the four groups. (I) Bile production in the first hour of reperfusion in each group. (J) Liver ICG metabolism in each group during the second hour of reperfusion. * p < 0.05 versus the CS I/R group, # p < 0.05 versus the HOPE I/R group. Scale bars: 50 μm (A) and 100 μm (E)