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. 2021 Nov 16;25(24):11310–11321. doi: 10.1111/jcmm.17062

FIGURE 7.

FIGURE 7

CIRP‐mediated oxidative stress and mitochondrial dysfunction in hepatocytes. HL‐7702 cells were treated with 100 ng/ml rhCIRP, 1000 ng/ml rhCIRP, or 1000 ng/ml rhCIRP +300 ng/ml C23 for 6 h (n = 3). (A) DHE fluorescence in the four HL‐7702 cell groups (magnification 400×). (B) Flow cytometry analysis of HL‐7702 cell apoptosis in the four groups. (C) Western blot analysis of NADPH oxidase and mitochondrial proteins. (D) DHE fluorescence of HL‐7702 cells in the four groups. (E) Apoptosis of HL‐7702 cells. The expression of inflammatory factors, including TNF‐α (F), IL‐6 (G), and IL‐1β (H) in the medium. *p < 0.05 versus the control group, # p < 0.05 versus the high rhCIRP group. Scale bars: 100 μm (A)