FIGURE 1.

Hypoxic stress leads to the induction of CA IX and/or XII mRNA expression in AML cells. Focussed quantitative RT‐PCR assays for CA I, II, IX and XII were carried out in (A) a diverse panel of AML cell lines as well as in (B) primary cells derived from FLT3/ITD⁺ AML patients with ND and R/R disease after exposure to 21% and 1% O₂ for 48 h. Results shown in (A) represent the mean ± SEM fold increase in mRNA expression versus the untreated control at 21% O₂ (n = 3). Due to the limited availability of human tissue, primary cell culture experiments (B) were only performed once for each patient sample