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. 2021 Nov 7;25(24):11198–11211. doi: 10.1111/jcmm.17040

FIGURE 1.

FIGURE 1

Conditioned medium (CM) from human or rat primary osteoblasts promoted proliferation in human lung cancer NCI‐H1299 and NCI‐H460 cells. A‐C, NCI‐H1299 and NCI‐H460 cells were cultured in αMEM, 1640,1640+αMEM (1:1), 1640+CM (1:1) from rat osteoblasts for 48 h, respectively. The cell viabilities were detected via MTT assay as described in the Material and methods section (A). The cell growth was detected via colony forming as described in the Material and methods section (B). The PCNA, Bcl‐2, Bid, c‐PARP and β‐actin protein levels were detected via Western blotting as described in the Material and methods section (C). D‐F, NCI‐H1299 and NCI‐H460 cells were cultured in F12, 1640,1640+F12(1:1), 1640+CM (1:1) from human osteoblasts for 48 h, respectively. The cell viabilities were detected via MTT assay as described in the Material and methods section (D). The cell growth was detected via colony forming as described in the Material and methods section (E). The PCNA, Bcl‐2 and β‐actin protein levels were detected via Western blotting as described in the Material and methods section (F). The data are representative of three independent experiments (**p<0.01, ***p<0.001, ****p<0.0001)