Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Standard Curve in real-time digital PCR. (A) Digital patterns for each microfluidic panel at increasing concentrations (770 reaction chambers per panel; 0.85 nL volume per chamber). (B) Standard curves correlating the Cq values with the concentration of each target; shaded blue area indicates the single-molecule region; shaded orange shows the bulk region; and the middle area displays the theoretical transition between the single-molecule and bulk.
