TABLE 2.
Standard curve parameter in real-time digital PCR.
| Target | Slope | Constant | Rsqra | Eff. (%)b | Single-molecule region | Transition region | Bulk region | ||
|---|---|---|---|---|---|---|---|---|---|
| 101 cp/pnl (occ.) c | 102 cp/pnl (occ.) c | 103 cp/pnl (occ.) c | 104 cp/pnl (occ.) c | 105 cp/pnl (occ.) c | |||||
| blaIMP | −2.953 | 37.875 | 0.978 | 118.111 | 7 (0.9%) | 51 (6.6%) | 519 (67.4%) | 770 (100.0%) | 768 (99.7%) |
| blaKPC | −3.354 | 38.275 | 0.993 | 98.661 | 5 (0.6%) | 56 (7.3%) | 398 (51.7%) | 769 (99.9%) | 770 (100.0%) |
| blaNDM | −3.705 | 40.62 | 0.996 | 86.174 | 4 (0.5%) | 21 (2.7%) | 190 (24.7%) | 767 (99.6%) | 769 (99.9%) |
| blaOXA-48 | −3.304 | 38.01 | 0.998 | 100.77 | 3 (0.4%) | 25 (3.2%) | 321 (41.7%) | 769 (99.9%) | 768 (99.7%) |
| blaVIM | −3.582 | 39.96 | 0.994 | 90.169 | 6 (0.8%) | 59 (7.7%) | 659 (85.6%) | 770 (100.0%) | 770 (100.0%) |
a
R-squared.
b
Efficiency (%).
c
Copies/panel (% occupancy in digital PCR). The occupancy is calculated by counting the number of amplification reaction occurring per each panel and diving it by the total number of wells (N = 770).