Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 23;12:752825. doi: 10.3389/fphar.2021.752825

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Hu, Qian, Wei, Jin, Kong, Cao and Ding.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

DSF/Cu inhibits CRC cell viability by inducing autophagy as revealed by CRISPR-Cas9 library screening. (A) Schematic diagram illustrating the workflow for genome-wide CRISPR-Cas9 knockout library screening (CRISPR: Clustered Regularly Interspaced Short Palindromic Repeats). (B,C) The distribution display of beta value of different genes in day 0 group and DSF treatment group for 7 (B) or 14 (C) days. Red points represent positive screening genes, and the blue ones are negative screening genes, (D) The positive screening genes related to autophagy. (E) qRT-PCR verified the expression of the upregulation autophagy-related genes in the control group and DSF/Cu. *p < .05; **p < .01; ***p < .001.