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. 2000 Dec;20(23):8748–8757. doi: 10.1128/mcb.20.23.8748-8757.2000

FIG. 5.

FIG. 5

Cyclin D1-cdk4 activity in p27/p21−/− MEFs. (A) Cell lysates of growing wild-type and p27/p21−/− MEFs were immunoprecipitated with antibody to cdk4 and immunoblotted with antibody to cyclin D1 (top panel) or were only immunoblotted with antibody to cyclin D1 (bottom panel). (B) Extracts of wild-type and cyclin D1-deficient MEFs were immunoblotted with antibody to cyclin D1. (C) Extracts of wild-type and p27/p21−/− MEFs were immunoprecipitated with antibody to cyclin D1 (lanes 1 and 4), preimmune serum (PI) (lanes 3 and 6), or cyclin D1 antibody that had been preincubated with the peptide against which the antibody was generated (lanes 2 and 5). Immune complexes were assayed for kinase activity using GST-Rb as substrate. Ab, antibody.