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. 2021 Nov 1;3(24):6876–6881. doi: 10.1039/d1na00696g

Calculation of conjugation efficiency (%), number of ligands by qPAINT and ligand availability (%) for PLGA–PEG NPs with varying maleimide content and spacer PEG lengthsa.

Maleimide content (%) Spacer PEG chain length (kDa)
Conjugation efficiency (%) Quantified ligands (qPAINT) Ligand availability (%)
5 1 5 1 5 1
10 25 38 6 42 8 21
20 21 35 8 54 6 15
30 31 70 31 79 2 5
a

PLGA–PEG NPs with increasing maleimide content (10–30%) and varying spacer PEG chain length (PEG5k and PEG1k). The conjugation efficiency (%) was calculated using spectrophotometry, the number of accessible ligands by qPAINT and the ligand availability (%) by considering the number of available ligands out of the theoretical expected number of ligands. For calculations of CE% and ligand availability see ESI Experimental section and Table S2 in ESI.