Skip to main content
. 2021 Nov 23;12:761418. doi: 10.3389/fgene.2021.761418

FIGURE 2.

FIGURE 2

Histological analysis and IHC demonstrating the increased cellular proliferation in the midbrain of Gpr161 cKO fetuses. (A) H&E staining of Gpr161 f/f and Gpr161 f/f ;Wnt1-Cre/+ at E13.5 (n = 3) and E15.5 (n = 3); cb, cerebellum; cp, choroid plexus; D, mandible; HP, hard palate; mb, midbrain; NC, nasal cavity; SP, soft palate; T, tongue; tel, telecephalon; X, maxilla (B) IHC with Ki-67 and pHH3 antibodies in midbrain sections of Gpr161 f/f and Gpr161 f/f ;Wnt1-Cre/+ at E13.5 (n = 3). The black boxes indicate the areas magnified in the right panel. VZ: ventricular zone, MZ: mitotic zone (C) The statistical analysis of IHC with Ki-67 and pHH3 shown in (B). Y axis indicates the percentage of neurons that were Ki-67, pHH3, and Gli1 positive. The experiments were done triplicate and values were shown as means and standard deviations (SD). The statistical analysis was performed using a 2-way ANOVA, followed by Tukey’s test for multiple comparison (GraphPad Prism 8).