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. 2021 Nov 23;12:761418. doi: 10.3389/fgene.2021.761418

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Copyright © 2021 Kim, Robles-Lopez, Cao, Liu, Chothani, Bhavani, Rahman, Mukhopadhyay, Wlodarczyk and Finnell.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The craniofacial skeletal analysis in Gpr161 cKO fetuses at E17.5. (A) The skeleton staining of heads from Gpr161 ^f/+ ;Wnt1-Cre/+ and Gpr161 ^f/f ;Wnt1-Cre/+ with Alcian blue and Alizarin Red S (n = 3). Fn: frontal bone; Pa: parietal bone; ip: interparietal bone; Na: nasal bone; Px: premaxilla; X: maxilla; D: mandible. (B) 3D reconstruction of microCT images of heads from Gpr161 ^f/f (n = 1), Gpr161 ^f/+ ;Wnt1-Cre/+ (n = 2) and Gpr161 ^f/f ;Wnt1-Cre/+ (n = 3) at E17.5. frontal bone: blue, parietal bone: yellow, interparietal bone: red, occipital bone: pink, nasal bone: purple, palatine bone: beige, maxilla: turquoise, premaxilla: green, mandible: orange (C) Quantitative volume measurement of identified craniofacial bones. Con combines the volume measurement from Gpr161 ^f/f and Gpr161 ^f/+ ;Wnt1-Cre/+.