Fig. 5.
(A) Semi-quantification of lipid content in the differentiated adipocytes treated with mitogenic concentrations of CQ-B (0.0001, 0.01, 1 and 10 μg/ml) determined by ORO staining method. The ORO content was quantified using ELISA reader. The ORO content is proportional to lipid content of the cells; therefore, comparison with the positive control (100%) gives relative lipid content of the cells. (B). Estimation of triglycerides in differentiated adipocytes treated with mitogenic concentrations of CQ-B (0.0001, 0.01, 1 and 10 μg/ml). The data are presented as Mean ± SD of technical and biological triplicates. (P≤0.05; ns = not significant, * P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001).