Table 1.
Macro- and microscopic phenotype descriptions of putative endophyte actinobacteria of native Chilean Solanum tuberosum subsp. tuberosum with their taxonomic identity according to 16S ribosomal gene analysis
| Isolate code | Origin of plant tissue | Culture media a | Aerial myceliab | Substrate myceliab | Soluble pigmentc | Filament diameter | Spore diameter | Spore disposition d | Identity by 16S ribosomal DNA partial gene | Closest taxa strain in NCBI | Nucleotide sequence | Similarity in Blast analysis | Accession in NCBI database | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ISP2 | ISP3 | ISP4 | |||||||||||||
| H2TP199 | Leaf | B | MB | MB | gray | orange | + | 0.77 um | 0.62 um | Rectiflexibiles | Streptomyces sp. | Streptomyces galilaeus strain P-YIM40 (GenBank: MT533915.1) | 1051 bp | 100% | KY242591 |
| G1TP199 | Root | MB | MB | B | gray | orange | + | < 0.5 um | < 0.5 um | ND d | Streptomyces sp. | Streptomyces sp. strain GF22 (GenBank: MN826279.1) | 1036 bp | 99.2% | KY242592 |
| KR31 | Stem | B | B | B | white | brown | + | 0.72 um | < 0.5 um | Retinaculiaperti | Streptomyces sp. | Streptomyces sp. 7R006 (GeneBank: LC497916.1) | 803 bp | 98.9% | KY242593 |
| DP143 | Tubercle | MB | MB | MB | white/gray | brown | + | 0.67 um | < 0.5 um | Retinaculiaperti | Streptomyces sp. | Streptomyces sp. YRA147 (GenBank: JX430828.1) | 1104 bp | 99.8% | KY242596 |
| A2R31 | Stem | MB | EX | B | gray | brown | + | 0.77 um | 0.89 um | Rectiflexibiles | Streptomyces achromogenes | Streptomyces achromogenes strain NBRC 12735 | 969 bp | 100% | KY242590 |
| CR34 | Tubercle | MB | B | B | NDe | cream | – | < 0.5 um | < 0.5 um | ND e | Nocardia sp. | Nocardia sp. FGT33 (GenBank: KU382293.1) | 750 bp | 99.9% | KY296348 |
| HP171 | Root | MB | MB | EX | white | orange | – | 0.67 um | 0.66 um | Rectiflexibiles | Streptomyces sp. | Streptomyces galilaeus strain PSWC-10 (GenBank: MT275786.1) | 1027 bp | 99.9% | KY242594 |
| MP136 | Leaf | MB | MB | MB | black | yellow | – | 0.56 um | 0.66 um | Spira | Streptomyces sp. | Streptomyces sp. CH9-7 (GenBank: LC489240.1) | 1078 bp | 99.8% | KY242595 |
| TP199 | Tubercle | EX | B | MB | gray | yellow | – | 0.56 um | < 0.5 um | Straight to flexuous chains | Streptomyces sp. | Streptomyces sp. KK13-6 (GenBank: LC487830.1) | 1147 bp | 99.6% | KY228978 |
| NP199 | Tubercle | MB | MB | MB | gray | yellow | – | 0.77 um | 0.58 um | ND e | Streptomyces sp. | Streptomyces sp. HST09 (GenBank: KX130883.1) | 1114 bp | 99.3% | KY242597 |
aCulture media according to [38], ISP2 (yeast extract and– malt extract agar), ISP3 (oatmeal agar) and ISP4 (inorganic salts and– starch agar). Furthermore, excellent (EX, 76 to 100%), very good (MB, 51 to 75%), good (B, 26 to 50%), poor (P, 1 to 25%), and negative (N, 0%)
bDetermined on ISP2 medium according to [38]
cNegative: -, Positive: +. Determined in ISP1 medium according to [38]
dDescription according to [39] e ND Not determined