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. 2021 Nov 23;12:766845. doi: 10.3389/fimmu.2021.766845

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Copyright © 2021 Xie, Li, Ye, Shan, Zhao, Duan, Liu, Unger, Cheng, Zhang, Wu and Xia

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Imaging analysis of immune cells in the hindgut of the zebrafish SBMIE larva model. (A) Hindgut innate immune cells, including neutrophils (lyz-DsRed labeled cells) and macrophages (mpeg-EGFP labeled cells); (B) the histogram of lyz or mpeg signals in the hindgut of larva. (C) Hindgut adaptive immune cells, including immature lymphocytes (rag2-DsRed-labeled red cells) and mature T lymphocytes (lck-EGFP-labeled green cells). (D) The histogram of rag2⁺ or lck ⁺ signals in the hindgut of larva. FM, fish meal diet; SBM, soybean meal diet; SN, sinomenine (35 ppm) supplementary SBM diet. The significant differences were indicated by p value, and *** represented p < 0.001. The scale bar in whole hindgut pictures was 100 μm, while the scale bar in the enlarged view was 20 μm.