Table 1.
Formulation of experimental diets.
| Raw Material g/kg | FM | SBM | SN |
|---|---|---|---|
| Fish meal | 555 | 250 | 250 |
| Soybean meal | 0 | 500 | 500 |
| Wheat meal | 255 | 110 | 110 |
| Starch | 50 | 50 | 50 |
| Fish oil | 30 | 60 | 60 |
| Mineral premix a | 10 | 10 | 10 |
| Vitamin premix b | 10 | 10 | 10 |
| Sinomenine | 0 | 0 | 0.7 |
| Cellulose | 90 | 10 | 9.3 |
| Gross weight (g) | 1,000 | 1,000 | 1,000 |
Per kilogram of mineral premix (g kg−1): MnSO4·H2O (318 g kg−1 Mn), 1.640g; MgSO4·H2O (150 g kg−1 Mg), 60.530 g; FeSO4·H2O (300 g kg−1 Fe), 23.110 g; ZnSO4·H2O (345 g kg−1 Zn), 0.620 g; CuSO4·5H2O (250 g kg−1 Cu), 0.010 g; KI (38 g kg−1 I), 0.070 g; NaSeO3 (10 g kg−1 Se), 0.005 g. All ingredients were diluted with cornstarch to 1 kg.
Per kilogram of vitamin premix (g kg−1): retinyl acetate (500,000 IU g−1), 2.40 g; cholecalciferol (500,000 IU g−1), 0.40 g; DL-a-tocopherol acetate (500 g kg−1), 12.55 g; menadione (230 g kg−1), 0.80 g; cyanocobalamin (10 g kg−1), 0.83 g; D-biotin (20 g kg−1), 4.91 g; folic acid (960 g kg−1), 0.40 g; thiamin hydrochloride (980 g kg−1), 0.05 g; ascorhyl acetate (930 g kg−1), 7.16 g; niacin (990 g kg−1), 2.24 g; meso-inositol (990 g kg−1), 19.39 g; calcium-D-pantothenate (980 g kg−1), 2.89 g; riboflavin (800 g kg−1), 0.55 g; pyridoxine (980 g kg−1), 0.59 g. All ingredients were diluted with corn starch to 1 kg.