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. 2021 Nov 23;12:766845. doi: 10.3389/fimmu.2021.766845

Table 2.

qPCR primers used in enteritis-related cytokine gene expression analysis.

Gene Name Forward sequence Reverse sequence
il-1β 5’-TGGACTTCGCAGCACAAAATG-3’ 5’-GTTCACTTCACGCTCTTGGATG-3’
nf-κb 5’-GATCATCGAGCAGCCTAAATC-3’ 5’-CCCACTGTAGTTGTGAACCCT-3’
tnf-α 5’-GCGCTTTTCTGAATCCTACG-3’ 5’-TGCCCAGTCTGTCTCCTTCT-3’
il-17a/f3 5’-AAGATGTTCTGGTGTGAAGAAGTG-3’ 5’-ACCCAAGCTGTCTTTCTTTGAC-3’
tgf-β1a 5’-TGTACCCGCAATCCTTGACC-3’ 5’-CCGACTGAGAAATCGAGCCA-3’
il-10 5’-CACTGAACGAAAGTTTGCCTTAAC-3’ 5’-TGGAAATGCATCTGGCTTTG-3’
il22 5’-GATGACTGATACAGCACGAAA-3’ 5’-CATTGATGCAGCAGGAACCT-3’
foxp3a 5’-GCCTCCATGATACGATGGGCAAT-3’ 5’-CCTTCCTTCAACACGCACAA-3’
rpl13a 5’-TCTGGAGGACTGTAAGAGGTATGC-3’ 5’-AGACGCACAATCTTGAGAGCAG-3’