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. 2021 Nov 23;9:762588. doi: 10.3389/fcell.2021.762588

FIGURE 4.

FIGURE 4

(A) The SRAMP database predicted the GGACU m6A site in hsa_circ_0058493. (B) MeRIP experiment shows that circNSUN2 is relatively enriched in m6A antibody compared with control IgG antibody. (C) When the hsa_circ_0058493 overexpression plasmid was transfected, the MeRIP experiment proved that the m6A antibody was more enriched. (D) Relative expression of METTL3 in 12 pairs of HCC and adjacent tissues was detected by qRT-PCR. (E) Hsa_circ_0058493 was positively correlated with METTL3. The P value and correlation coefficient were calculated by Pearson correlation analysis. (F) The knockdown efficiency of the METTL3 knockdown plasmid was tested by qRT-PCR. (G) MeRIP experiment proved that after knocking down METTL3 in hsa_circ_0058493, the degree of m6A methylation decreased. *P < 0.05, ***P < 0.001 vs. control group.