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. 2021 Nov 23;9:762588. doi: 10.3389/fcell.2021.762588

FIGURE 5.

FIGURE 5

(A) A CCK-8 assay was used to determine the proliferation of HCC cells after transfection with si-NC + oe-NC, si-YTHDC1 + oe-NC, and si-YTHDC1 + oe-circ. (B) A cloning assay was performed to detected proliferation ability of HCC cells with si-NC + oe-NC, si-YTHDC1 + oe-NC, and si-YTHDC1 + oe-circ. Colony formation rates are shown by histograms. (C) The cell cycle of HCC cells after transfection with si-NC + oe-NC, si-YTHDC1 + oe-NC, and si-YTHDC1 + oe-circ was determined by flow cytometry. As evident in the histogram, more cells arrested in the G1 phase were observed in si-YTHDC1 + oe-NC compared with si-NC + oe-NC. After cotransfection of si-YTHDC1 + oe-circ, the number of cells arrested in G1 phase decreased again compared with si-YTHDC1 + oe-NC. The triangle symbols are for discrimination of G1 vs. S and S vs. G2. (D) The invasion and migration abilities of HCC cells after transfection with si-NC + oe-NC, si-YTHDC1 + oe-NC, and si-YTHDC1 + oe-circ were assessed by Transwell assay. Histograms were used to show the number of invaded and migrated cells. *P < 0.05, **P < 0.01 vs. control group.