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. Author manuscript; available in PMC: 2022 Jul 30.
Published in final edited form as: Clin Sci (Lond). 2021 Jul 30;135(14):1773–1789. doi: 10.1042/CS20210549

Figure 4. ET-1 is up-regulated in adipose tissue via Hif1α.

Figure 4.

(A) Hif1α mRNA, (B) Pre-pro ET-1 mRNA, (C) ET-1 protein, (D) EdnrA mRNA, and (E) EdnrB mRNA from eWAT of mice after 10 weeks on diet and 2 weeks of treatment with atrasentan or bosentan were determined via ddPCR (n=5 per group). (F) Hif1α and (G) Pre-pro ET-1 gene expression from 3T3-L1 adipocytes exposed to 6 h of normoxia or hypoxia in the presence of Hif1α inhibitor IDF-117744 (1, 25, 50 μM) (n=6). Data from (A–E) were analyzed by one-way ANOVA with Tukey’s post-hoc test for individual groups. (F,G) were analyzed by two-way ANOVA. Data are expressed as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.