FIGURE 1.

Low‐uracil growth relies on the Fur4 transporter. (A) Schematic illustration showing uracil‐auxotroph cells (ura3∆) with (left) and without (right) the uracil permease Fur4. (B) Indicated yeast strains were grown to mid‐log phase before plating on rich (YPD) and synthetic complete (SC) media containing either 4 or 0.1 mg/L uracil. (C) Quantification of yeast growth from (B), asterisks (*) indicate Student t test comparisons P = <.001. (D) Wild‐type cells expressing Fur4‐mNeonGreen (Fur4‐mNG) from its endogenous promoter were labelled with FM4‐64 for 1‐hour, grown to mid‐log phase in SC‐Ura media (upper) or in the presence of 40 μg/mL uracil for 1 hour (lower) prior to confocal microscopy. Arrows indicated plasma membrane (PM), scale bar = 5 μm