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. 2021 Nov 23;12:740177. doi: 10.3389/fpls.2021.740177

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Copyright © 2021 Luo, Meng, Tan, Yin and Luo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Establishment of the RPA/Cas12a-fluorescence assay. (A) End point of Cas12a-fluorescence assay at different LbCas12a/crRNA ratios. (B) Optimization of LbCas12a concentration. (C) Optimization of FQ-reporter concentration. (D) Visualization with a mini-UV torch. (E) Optimized visualization results of FQ-reporter with a mini-UV torch. The UV light images were taken by the gel imaging analysis system.