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. 2021 Nov 23;12:740177. doi: 10.3389/fpls.2021.740177

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Copyright © 2021 Luo, Meng, Tan, Yin and Luo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The RPA/Cas12a-LFA assay. (A) Schematics of RPA/Cas12a-LFA assay. (B) The preliminary establishment of the RPA/Cas12a-LFA system. (C) Sensitivity of the RPA/Cas12a-LFA assay. (D,E) Specificity of the RPA/Cas12a-LFA assay. (F) Field detection of the RPA/Cas12a-LFA assay. BC, blank control.