FIGURE 2.

The ability of CAT-PEG to reduce ROS level, prevent cell injury and to regulate the production of inflammatory factors. (A) Fluorescence images of HPAEpi cells after incubation with CAT-PEG for 12 h followed by adding 1 mM H₂O₂ and 10 μM DCFH-DA and incubating for 30 min (green: ROS, blue: nuclei). Scale bar: 50 μm. (B) Relative fluorescent intensity of DCFH-DA in the HPAEpi cells after incubation with CAT-PEG for 12 h followed by adding 1 mM H₂O₂ and 10 μM DCFH-DA and incubating for 30 min (% of Control group). (C) Cell viability of the HPAEpi cells with 8 μg/ml, 16 μg/ml, 40 μg/ml of CAT-PEG for 12 h, followed by addition of H₂O₂ (1 mM) and culturing for 24 h (D, E) The concentration of TNF-α (D) and IL-6 (E) in the media of HPAEpi cells cultured with WBC, LPS, and different concentrations of CAT-PEG. In the Pre-addition study, HPAEpi cells were first cultured with different concentrations of CAT-PEG for 12 h, followed by adding LPS (1 μg/ml) and culturing for 24 h. In the Post-addition study, HPAEpi cells were cultured with 1 μg/ml LPS for 24 h, followed by culturing in fresh media containing different concentrations of CAT-PEG for 12 h n = 3. Data represent means ± s.e.m., p value: *p＜0.05, **p＜0.01, ***p＜0.001 and ****p＜0.0001. ns means not significant.