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. 2021 Nov 23;9:800684. doi: 10.3389/fbioe.2021.800684

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Copyright © 2021 Li, Yan, Wu, Han, Qin, Liu and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histologic analysis of the control, model, and treatment group. (A) Representative H and E staining sections of the major organs (Original Magnification ×200). (B) Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining of the liver, kidney, and lung sections. Scale bar: 50 μm. The nucleus was stained with DAPI (blue). Apoptotic cells were stained showing green color. (C–E) The percentage of TUNEL positive cells, which was counted by Leica TCS SP8 Confocal laser scanning microscope for each section and counted at least three viewing fields in each experiment. The tissue from top to bottom: liver, kidney, and lung. n = 3. Data represent means ± s.e.m. *p＜0.05 and **p＜0.01. ns means not significant.