Figure 3.
Neither B. mallei nor B. pseudomallei K96243 suppress activation of TLR2 or TLR4 at 37°C. B. mallei (A) and B. pseudomallei K96243 (B) were grown at 28°C or 37°C for ~48 h, and bacterial suspensions were diluted in PBS to inoculate HEK293-TLR2 or -TLR4 cells. Media controls contained only PBS rather than bacterial cells and positive controls contained either HKLM cells (2 x 106 cells) for TLR2 or E. coli LPS (0.2 ng) for TLR4. All samples were analyzed in triplicate, and results represent one of two independent repeats. Samples were read at 630 nm after ~20 h of incubation. Results are presented as geometric mean with standard error of the mean. Significant values compared to media control are shown: *P ≤ 0.05; **P ≤ 0.0100; ***P ≤ 0.0010; *****P ≤ 0.00001.