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. 2021 Nov 23;11:771524. doi: 10.3389/fcimb.2021.771524

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Copyright © 2021 Khandelwal, Chander, Kumar, Nagori, Verma, Mittal, T, Kamboj, Verma, Khatreja, Pal, Gulati, Tripathi, Barua and Kumar

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SARS-CoV-2 life cycle. Confluent monolayers of Vero cells, in triplicates, were infected with SARS-CoV-2 at MOI of 5 and thereafter washed with PBS, and fresh MEM was added. Infectious progeny virus particles released in the infected cell culture supernatant at indicated time points were quantified by plaque assay. One-step growth curve of WT (A) and Delta variant (B) of SARS-CoV-2 is shown. Statistical comparisons of viral titers (WT versus Delta SARS-CoV-2) were performed at 6 hpi (when progeny virus particles start appearing in the infected cell culture supernatant) and at 24 hpi (when Delta virus is about to complete its life cycle) by two-tailed Student’s t-test (C). **p < 0.01, ***p < 0.001.