Figure 2.

Secretion of inflammatory proteins after stimulation with LAM, PIM, and PPD. Culture supernatants were assessed for 92 protein markers using the Olink inflammation panel after 24 h of culture. Only markers above background levels were included (n=58). The graphs show normalized protein expression (ΔNPX) for stimulated (LAM, PIM, or PPD) minus unstimulated (PBS) PBMCs (A, n=15/group) and separated into individuals with active TB (ATB; red), latent TB (LTB, blue), and healthy controls (HC, black) (B, n=5/group). The lines indicate the mean and error bars indicate SEM. The protein markers were grouped into different functional groups (yellow highlight), where GF correspond to growth factors. Statistics was evaluated using two-way ANOVA followed by Dunnet’s posthoc test with significant differences between ATB or LTB against HC indicated by #, corresponding to a p-value < 0.05.