Figure 3.

Stimulation-induced cytokine production. (A) Representative gates for identification of cytokine producing CD45⁺ cells via intracellular staining. (B) The frequency of cytokine positive cells out of CD45⁺ cells for each stimulation at 24h. All donors merged (n=15/stimulation). Statistics was evaluated by Friedman’s test with Dunnet’s posttest where every group was compared with unstimulated cells (PBS). *p<0.05, **p<0.01, ****p<0.0001. (C) For each cytokine, the pie-charts indicate the frequency of cell type responsible for its production: B cells (blue), myeloid cells (red), NK cells (green), T cells (yellow), and others (grey).