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. 2021 Nov 23;12:727300. doi: 10.3389/fimmu.2021.727300

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Copyright © 2021 Silva, Sundling, Folkesson, Fröberg, Nobrega, Canto-Gomes, Chambers, Lakshmikanth, Brodin, Bruchfeld, Nigou, Correia-Neves and Källenius

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-6 production by myeloid cells upon PIM stimulation is regulated via TLR2. Dot plots of IL-6 gating within myeloid cells (top) and percentage of cells producing IL-6 within myeloid cells (bottom). PBMCs were pre-treated with anti-TLR2 or isotype control and then stimulated with PIM, LAM, and PHA during 24 h. IL-6 production was evaluated by flow cytometry. Statistical differences between anti-TLR2 and isotype were evaluated by a paired t test (n=9) ***p<0.001. NC, negative control. The red and black colors correspond to two independent experiments.