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. Author manuscript; available in PMC: 2022 Sep 8.
Published in final edited form as: Cell Host Microbe. 2021 Aug 11;29(9):1421–1436.e7. doi: 10.1016/j.chom.2021.07.006

Figure 3. tRNA determinants for MA binding.

Figure 3.

(A & B) Schematic representations of MA-tRNALys3 interactions at the elbow (A) and inner corner (B). The T-loop motif (t54-t58) is shown in light green.

(C) Mutational and ITC analyses of human tRNALys3, tRNAGly, tRNAPhe, tRNAThr, tRNALeu and tRNASer. Data are represented as mean ± s.d.

(D) Secondary structures of the elbow regions of the six tRNAs assayed in (C). Red crosses denote deletion.

(E) Representative ITC isotherms of MA binding to the six tRNAs in (D). The error bars indicate uncertainties in individual injection heats estimated by NITPIC (Methods).

See also Figure S2, S3, S4, Table S2.