KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat anti-gp120 HIV-1 | ARP | Cat#12–6205 RRID:AB_1541516 |
| Anti-HIV-1 p24 (183-H12–5C) | NIH AIDS Reagent Program | Cat#3537 RRID:AB_2832923 |
| Human anti-HIV-1 gp120 Monoclonal 2G12 | Polymun Scientific | Cat#AB002 RRID:AB_2661842 |
| IRDye800CW Donkey anti-Goat IgG | LI-COR Bioscience | Cat# 926–32214 RRID:AB_621846 |
| IRDye 680RD Donkey anti-Mouse IgG | LI-COR Bioscience | Cat# 926–68072 RRID:AB_10953628 |
| Goat anti-Mouse IgG - Atto 594 | Sigma | Cat# 76085 RRID:AB_1137653 |
| Abberior STAR RED, donkey anti-human IgG | Abberior | Cat# STRED-1054 RRID:AB_2877172 |
| Bacterial and Virus Strains | ||
| BL21-CodonPlus (DE3)-RIL Competent Cells | Agilent | Cat# 230245 |
| Biological Samples | ||
| LeukoPaks – Buffy coats made from human whole blood | NYBC, New York | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| T4 RNA ligase 1 | New England BioLabs | Cat#M0204S |
| Human recombinant IL-2 | PeproTech | Cat#200–02 |
| Human recombinant GM-CSF | PeproTech | Cat#300–03 |
| Critical Commercial Assays | ||
| QuikChange Lightning | Agilent | Cat#210519 |
| Deposited Data | ||
| Atomic coordinates and structure factor amplitudes for the co-crystal structure of HIV-1 MA bound to human tRNALys3 | This paper | PDB: 7MRL |
| Experimental Models: Cell Lines | ||
| HeLa | ATCC | Cat#: CCL-2 RRID:CVCL_0030 |
| 293T | ATCC | Cat#CRL-3216 RRID: CVCL_0063 |
| SupT1 | ATCC | Cat# CRL-1942 RRID: CVCL_1714 |
| MT4-LTR-GFP | (Kane et al., 2016) | N/A |
| CEM X174 | NIH AIDS Reagent Program | Cat#234–220 RRID: CVCL_X615 |
| Oligonucleotides | ||
| 5′ RNA oligo to anneal to form tRNALys3 with 2Ap10 5′-rGrCrCrCrGrGrArUrArGrCrUrCrArGrUrCrG/i2AmPr/rUrA rGrArGrCrArUrCrArGrArCrU-3′ |
Integrated DNA Technologies | N/A |
| 3′ RNA oligo to anneal to form tRNALys3 with 2Ap10 /5′Phos/rUrUrUrArArUrCrUrGrArGrGrGrUrCrCrArGrGrGrUrUrCrArArGrUrCrCrCrUrGrUrUrCrGrGrGrCrGrCrCrA-3′ |
Integrated DNA Technologies | N/A |
| 5′ RNA oligo to anneal to form tRNALys3 with 2Ap19 5′ -rGrCrCrCrGrGrArUrA/i2AmPr/rCrUrCrArGrUrCrGrGrUrA rGrArGrCrArUrCrArGrArCrU-3′ |
Integrated DNA Technologies | N/A |
| 3′ RNA oligo to anneal to form tRNALys3 with 2Ap19 /5′Phos/rUrUrUrArArUrCrUrGrArGrGrGrUrCrCrArGrGrGrUrUrCrArArGrUrCrCrCrUrGrUrUrCrGrGrGrCrGrCrCrA-3′ |
Integrated DNA Technologies | N/A |
| Forward primer to amplify MAK32A fragment from pNL4–3(MAK32A) 5′-CCA GAG GAG ATC TCT CGA C-3’ and 5’-CCC CTT GGT TCT CTC ATC-3′ |
Integrated DNA Technologies | N/A |
| Reverse primer to amplify MAK32A fragment from pNL4–3(MAK32A) 5′-CCC CTT GGT TCT CTC ATC-3′ |
Integrated DNA Technologies | N/A |
| Forward primer to amplify the LacZ fragment from pLHCX-GFP-LacZ 5′-CTCGGCATGGACGAGCTGTACAAGTCCGGAGCCGTCGTTTTACAAC-3′ |
Integrated DNA Technologies | N/A |
| Reverse primer to amplify the LacZ fragment from pLHCX-GFP-LacZ 5′-GCTGATCAGCGGGTTTAAACGGGCCCTCTAGATTTTTGACACCAGACCAACTGGTAATGG-3′ |
Integrated DNA Technologies | N/A |
| Additional oligos for mutational analyses can be found in Supplemental Table 4 | Integrated DNA Technologies | N/A |
| Recombinant DNA | ||
| pET19b-MA | (Gaines et al., 2018b) | N/A |
| pNL4–3 | (Adachi et al., 1986) | N/A |
| pNL4–3(MAK32A) | This paper | N/A |
| pNL/YU2 | (Zhang et al., 2002) | N/A |
| pNL/ADA | (Zhang et al., 2002) | N/A |
| pNL/BAL | (Mariani et al., 2001) | N/A |
| pNL/YU2(MAK32A) | This paper | N/A |
| pNL/ADA(MAK32A) | This paper | N/A |
| pNL/BAL(MAK32A) | This paper | N/A |
| pCR3.1coGag-GFP | (Jouvenet et al., 2006) | N/A |
| pCR3.1coMAK32A-GFP | (Jouvenet et al., 2006) | N/A |
| pCR3.1coMA-3xHA | This paper | N/A |
| pCR3.1coGag(MAK32A)-GFP | This paper | N/A |
| pCR3.1coMAK32A-GFP | This paper | N/A |
| pCR3.1coMAK32A-3xHA | This paper | N/A |
| pLHCX-GFP-LacZ | (Kane et al., 2016) | N/A |
| pCR3.1coMAK32A-GFP-LacZ | This paper | N/A |
| pVSV-G | (Yee et al., 1994) | N/A |
| Software and Algorithms | ||
| SEDPHAT | (Zhao et al., 2015) | RRID:SCR_016254 http://www.analyticalultracentrifugation.com/sedphat/ |
| NITPIC | (Keller et al., 2012) | https://www.utsouthwestern.edu/labs/mbr/software/ |
| SEDFIT | (Schuck, 2000) | RRID:SCR_018365 https://sedfitsedphat.nibib.nih.gov/software/default.aspx |
| REDATE | (Zhao et al., 2013b) | https://www.utsouthwestern.edu/labs/mbr/software/ |
| SEDNTERP | (Cole et al., 2008) | RRID:SCR_016253 http://www.jphilo.mailway.com/sednterp.htm |
| ASTRA | Wyatt Technology Europe | RRID:SCR_016255 https://www.wyatt.com/products/software/astra.html |
| Phaser | (McCoy et al., 2007) | RRID:SCR_014219 https://www.phenixonline.org/documentation/reference/phaser.html |
| Phenix | (Liebschner et al., 2019) | RRID:SCR_014224 https://www.phenixonline.org |
| Coot | (Emsley et al., 2010) | RRID:SCR_014222 https://www2.mrclmb.cam.ac.uk/personal/pemsley/coot/ |
| ERRASER | (Chou et al., 2013) | https://rosie.graylab.jhu.edu/erraser |
| Other | ||
| GraphPad Prism | GraphPad Software | RRID:SCR_002798 https://www.graphpad.com |
| Origin | OriginLab Corporation | RRID:SCR_014212 https://www.originlab.com/ |
| FIJI | (Schindelin et al., 2012) | RRID:SCR_002285 https://fiji.sc |
| DeltaVision SoftWoRx | GE Healthcare | RRID:SCR_019157 https://www.gehealthcare.com |
| Imspector Image Acquisition & Analysis Software | Abberior Instruments Development Team | RRID:SCR_015249 http://www.imspector.de |
| Huygens STED Deconvolution Software, Huygens Professional | SVI | RRID:SCR_014237 https://svi.nl/Huygens-STED-Software |