TABLE 1.
Protein | sNrk/s-dRor2 | sDrl-2 |
---|---|---|
PBD ID | 7ME4 | 7ME5 |
Crystallization Conditions | 3 mg/ml protein, 50 mM Bis-Tris propane (pH 5.0), 20% PEG 3350, 21°C | 12 mg/ml protein, 100 mM Tris (pH 8.5), 100 mM sodium acetate, 25% PEG 6000, 15% glycerol, 21°C |
Data Collection a | ||
Source | APS 24-ID-E | Rigaku 007HF |
Wavelength (Å) | 0.9792 | 1.5418 |
Space Group | C2 | C2221 |
Cell Dimensions | ||
a, b, c (Å) | 95.70, 74.69, 61.55 | 56.97, 91.58, 76.36 |
α, β, γ (°) | 90, 106.31, 90 | 90, 90, 90 |
Resolution (Å) | 45.61 – 1.75 | 50.00 – 2.0 |
Completeness (%) | 88.5 (78.0) | 99.53 (94.2) |
Redundancy | 2.2 (1.8) | 6.6 (3.6) |
Rsym | 0.046 (0.744) | 0.061 (0.769) |
I/σ | 9.1 (1.0) | 19.1 (1.5) |
CC1/2 | 0.998 (0.630) | 0.999 (0.597) |
Refinement | ||
Number of reflections | 36,909 | 13,749 |
Rwork/Rfree (%) | 19.9/22.2 | 21.8/24.8 |
Number of atoms | ||
Protein | 1,833 | 1,209 |
Ligands | 18 | - |
Carbohydrate | 28 | 14 |
Water | 275 | 84 |
Average B factor (Å) | ||
Protein | 41.95 | 25.26 |
Ligands | 75.53 | - |
Carbohydrates | 63.49 | 90.50 |
Water | 49.26 | 52.30 |
Ramachandran favored (%) | 97.7 | 99.3 |
Ramachandran allowed (%) | 2.3 | 0.7 |
Ramachandran outliers (%) | 0 | 0 |
Bond length rmsd (Å) | 0.009 | 0.006 |
Bond angle rmsd (Å) | 0.950 | 0.784 |
Numbers in parentheses denote highest resolution shell